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Electrochemical and spectroscopic results confirm that artemisinin can intercalate into DNA double helix, which causes DNA conformational changes.
It can be concluded that DNA conformational changes are determinant in the transduction process and that the electrostatic effect is negligible.
Therefore, this method can provide stable plasmid DNA network that may be useful to manipulate nanostructure using typical plasmid DNA conformational changes: supercoiled circular and open circular forms.
Ferrocene (Fc), being labeled on single stranded DNA as one signal indicator, could provide "turn on" signal through the DNA conformational changes resulted from target binding with aptamer.
Thus, controlling these DNA conformational changes to acquire the desired function is significant for evolving DNA hybridization biosensor, used in genetic screening and molecular diagnosis.
After discarding DNA conformational changes at 5NNN region that could have affected the configuration of the active site, we analysed the crystal structures of both I-CreI_D75N and I-CreI_3115 proteins in complex with cleavable and non-cleavable DNA target sequences and in the presence of the catalytic ion Mg2+ in order to decipher the molecular basis of this different behaviour.
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DNA conformational change and breakage induced by artemisinin, a traditional Chinese herbal medicine, have been visually characterized and quantitatively measured by the multiple tools of electrochemistry, UV vis absorption spectroscopy, atomic force microscopy (AFM), and DNA electrophoresis.
Alternatively, histone replacement could be initiated by a local, ATP-driven DNA conformational change near SHL2 that alters the path of the DNA superhelix, resulting in destabilization of H2A-H2B contacts with DNA or with the H3-H4 tetramer.
Finally, Ku70/Ku80 heterodimers and DNA induce conformational changes in the FAT domain of DNA-PKcs and enhance its kinase activity (Spagnolo et al, 2006).
Our approach allowed us to monitor fast blinking of an organic dye, the dissociation kinetics of very short DNA and conformational changes of biomolecules with much improved temporal resolution than the conventional method.
The Elliomut gel is a specially formulated non-denaturing polyacrylamide gel that allows the separation of single stranded DNA by conformational changes and also analysis of the heteroduplex.
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