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The unusual structures reported here are consistent with both the prediction [42] and experimental evidence [43] that DNA composed of long stretches of CAG repeats is particularly flexible.
An 87-nucleotide synthetic DNA composed of 21 and 16 nucleotides flanking a "variant region" of 50 random nucleotides (5'-CGGAATTCCGAGCGTGGGCGT (N 50 TACGCCCACGCTCGAG-3' (87 mer)), was generated and purified by HPLC (Integrated DNA Technologies Inc., Coralville, IA, USA).
The total genomic DNA (composed of fragments ranging in size between 40 and 100 kb) from the different plasmid-transformed R. solanacearum GMI1000 clones was used as donor DNA with GMI1000, CFBP2968, NCPPB332 and CFBP2957 as recipient strains.
SSRs are tandemly repeated tracts of DNA composed of 1 6 base pair (bp) long units.
The pGA2/JS7 DNA vaccine is a 9.5 kb plasmid DNA composed of a 2.9 kb expression vector termed pGA2 and a 6.6 kb vaccine insert termed JS7.
Digestion of the ligated DNA with Sfi I enzyme linearizes circular DNA composed of truncated 5' part of the insertion vector and a genomic fragment, and generates a unique overhang on the λ buffer of vector for ligating splinkerette adaptor.
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In Arabidopsis, the T-DNA integration occurs between the recipient genome and microhomologous sequences of the T-DNA composed of 5-bp and 2-bp sequences on the left border and right border, respectively (Brunaud et al. 2002).
Sanchez et al. (2009), in an effort to develop genomic tools for the Rainbow trout (Oncorhynchus mykiss), performed WGS on a pool of genomic DNA composed out of 96 unrelated rainbow trout.
Non-coding regions harbor plenty of functional DNA, composed essentially of regulatory elements such as enhancers, promoters, insulators and silencer, and of non-coding functional RNA such as micro-RNA (miRNA).
Their mitochondrial DNA, termed kinetoplast (k) DNA, is composed of dozens of maxicircles and thousands of minicircles, mutually interlocked into a single giant network (Liu et al. 2005).
The resulting circles are then amplified in solution into coils of single-stranded DNA [ 31], called DNA nanoballs, composed of repeated copies of the original circles.
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