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DNA complexes of spermine and spermidine become resolubilized at very high concentrations of the oligoamine.
The thermal stability of the DNA complexes of apaMyoD was increased by 13°C relative to MyoD-bHLH.
CNS gene transfer mediated by a novel controlled release system based on DNA complexes of degradable polycation PPE-EA: A comparison with polyethylenimine/DNA complexes.
The structures of AbrB, and DNA complexes of Rob and MuR unveil a novel DNA-binding motif, 'the looped-hinge helix', and new uses of the helix-turn-helix and winged helix motifs in DNA binding.
To obtain initial affinity values for the DNA complexes of different compounds, thermal melting experiments were performed with hairpin oligomer duplexes containing a pure AT sequence (5′-AATT-3′) and a mixed sequence (5′-ATGA-3′ in the preferred 5′-ATGAT-3′ context).
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Researchers at UCSF and Kaiser Permanente studied the telomere length (DNA complexes on the end of chromosomes) in 110,000 participants.
In a DNA complex of c-Myb, both the second and third repeats are closely packed in the major groove of DNA and recognize a specific base sequence cooperatively.
Adding increasing amount of wild-type phiC31 integrase to the reaction leads to the disappearance of the free-probe band, while three protein-DNA complexes of retarded electrophoretic mobility can be observed, referred as to I, II, and III, and the major species obtained corresponding to the complex II (Fig. 4A and 4B).
Incubation of the MMP-1 probe with nuclear extracts from untreated fibroblasts led to the formation of two protein-DNA complexes, of which only the upper band was modulated by treatment (gray arrow), demonstrating the binding of one or several transcription factors to this synthetic DNA sequence.
The culture medium was replaced with free-FBS RPMI-1640 medium before the sequential addition of 0.1 ml protein-DNA complexes of TfRscFv-GAL4 and GAL4rec-GFP-pGes, after incubation for 5h in 5% CO2 at 37°C, 1 ml of medium with 10% FBS was added and incubated for another 48h.
Immobilization of DNA complexes to tissue culture polystyrene substrates can be a useful tool for enhancing gene delivery for in vitro studies.
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