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For in vivo application, DNA complexes have to pass a variety of anatomical and physiological barriers, and an environment of biological fluids and extracellular matrix before reaching their targets.
Recently, nitrogen-core poly(propyl ether imine) (PETIM) dendrimer DNA complexes have been investigated and results showed low toxicities and efficient gene delivery vector properties.
DNA complexes have been made that change their conformation upon some stimulus, making them one form of nanorobotics.
Various structures of HMG-domain DNA complexes have shown that the structure of the HMG-core is maintained upon DNA binding.
Cationic lipid DNA complexes have been used as a non-viral gene delivery system in cell culture [ 1- 5] and in several animal models [ 6- 11].
Previous structural and binding studies of NF-κB:κB DNA complexes have provided some mechanistic insights into how κB sequences affect affinity and conformation.
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Molecular diagnostic assays, such as nested polymerase chain reaction and a species-specific DNA probe from the ribosomal DNA complex, have been useful to identify P. insidiosum in the absence of culture (8, 9).
Structural studies of protein-DNA complexes have shown that there are many distinct families of DNA-binding proteins, and have shown that there is no simple "code" describing side-chain/base interactions.
3D-structures for a number of mammalian HMG-DNA complexes have been determined, including Sox2 [28] used in Figure 4, HMG-D [37], LEF-1 [38] and SRY [30].
Consistent with a role for MRE11 in promoting cleavage of tyrosyl-DNA covalent bonds, topoisomerase-DNA complexes have been shown to persist in an S. pombe rad32(mre11 -D65N mre11 -D65Nowing treatment with CPT or ETP [27].
TOP1 and TOP2-DNA complexes have been observed following Curcumin administration to K562 cells.
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