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The cells were incubated in the presence of DNA alone or DNA complexes for 4 h and further 44 h after the removal of DNA or DNA complexes, and then observed by fluorescence microscope.
Consequently, it has been demonstrated that the interactions between various DNA complexes and GAGs are far from identical.
Figure S9 shows how similar most dendrimer complexes were in the stability of their DNA complexes and DNA unloading capabilities, dissociating at only slightly different heparin concentrations.
Extracellular polyanionic GAGs might bind the positively charged DNA complexes and, thus, disturb their mobility in tissue, as well as interaction with target cells.
The SC based gene therapy using CD suicide therapy showed cytotoxic effects which are based on three properties: (a) formation of (5-FU) RNA, (b) 5-FU DNA complexes, and (c) thymidylate synthase [ 3].
We measured the salt concentration dependences of the thermodynamic and kinetic parameters for formation of both nonspecific and specific DNA complexes and the effect of salt on steady-state catalysis by hUNG.
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However, in actively dividing cells the high number of collisions may exceed the capacity of the cells to eliminate TOPI-DNA complexes and the DNA repair capability of the cells and, under these circumstances, cell death is initiated.
In particular, DNR intercalates the base pairs forming DNR-DNA complexes and inhibits the activity of the enzyme topoisomerase II by stabilizing the DNA-topoisomerase II complex, preventing the enzyme-catalysed reaction.
The diblock copolymer-DNA complexes induced higher gene expression than the brush-like block copolymer-DNA complexes, and the statistical copolymer-DNA complexes mediated much lower gene expression than the block-like copolymers-DNA complexes.
EXPERT OPINION: Recent research has identified several novel mediators of thrombus formation, including cell-free nucleic acids, histones, histone-DNA complexes and neutrophil extracellular traps that may serve to link inflammation and thrombosis as well as separate physiological hemostasis from pathological thrombosis.
Cells were exposed to formaldehyde to cross-link protein-DNA complexes and processed as previously described [11], [46].
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