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In a typical experiment, SS DNA was hybridized with PC linker-modified LS DNA (sequences are shown in Table S1) to form a DNA complex with two single-strand tails.
Thus, the DNA complex with LD L22P) appears to provide a closer approximation of the wild-type structure.
With increased UV irradiation time, the bands with small migration became less intense, but the bands of the newly formed DNA complex with one tail became more intense.
As indicated in Figure 1 c, the band with the small migration in all lanes corresponded to the DNA complex with two single-strand tails.
Conversely, the band with large migration in all lanes corresponded to the newly formed DNA complex with one tail after UV irradiation.
The core of the liposome was an asODN or plasmid DNA complex with DOTAP (1 20 w/w) while the bilayer coat was formed of HPC, DC-CHOL, DOPE, DSPE-PEG, in a ratio of 0.5 0.2 0.1 0.2 (w/w).
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The cells were washed once with serum-free medium and incubated with medium containing DNA, DNA-peptides corplex, or DNA complexed with cationic lipids (DOSPA/DOPE) for 8 h at 37°C.
To determine the exact amount of cleaved PC linker, the band intensities of DNA complexes with two single-strand tails and newly formed DNA complexes with one single-strand tail after different UV irradiation times were analyzed with ImageJ software.
Crystal structures of DNA complexes with two BER proteins (MutY and EndoIII) revealed the proximity of these clusters to the DNA backbone.
At a neutral charge ratio, DNA complexes with PEI were polydisperse and substantially aggregated, whereas DNA complexes with PEI-g-1PEG-RGD were homogeneous with 100 200 nm effective diameter.
Substrate-mediated delivery describes the immobilization of DNA, complexed with cationic lipids or polymers, to a biomaterial or substrate.
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