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DNA coding for a peptide is frequently chemically synthesized while DNA coding for larger proteins may also be purified from natural sources.
In initial studies, BALB/c mice were immunized by intramuscular injection with DNA coding for human survivin (pcDNA3.1/hSurv).
Methods: C57BL/6 mice were immunized with DNA coding for human gp100, mouse gp100, or control vector by gene gun.
Conclusions: Immunization with xenogeneic DNA coding for the melanosomal glycoprotein gp100 results in tumor protection and autoimmune depigmentation.
Ironically, the initial idea of genes as simple stretches of DNA coding for a protein became dissolved in this process.
This interference with the intercellular signaling stimulates the GA3 activity and promotes the DNA coding for the synthesis of alpha amylase enzyme in the aleurone cell layer.
We hypothesized that immunizing mice with xenogeneic DNA coding for the human melanosomal membrane glycoprotein gp100 would overcome immune ignorance or tolerance and result in tumor immunity.
Novel forms of knockout and knockdown animals are now being developed in which regions of DNA coding for miRNAs are being deleted or their expression reduced, respectively.
They carry their own DNA, coding for 37 genes, which is passed down from mother to child through the mitochondria in the egg cytoplasm.
They injected DNA coding for a bacterial phytase gene into more than 4000 pig embryos, 1% of which incorporated the gene.
The DNA coding for crtW was inserted into the Sfu I and EcoR I restriction sites of plasmid pGAPZαA, and the same restriction sites were used for inserting crtZ DNA into pGAPZαA*.
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