Sentence examples for dna cloning methods from inspiring English sources

Simple and low-cost recombinant enzyme-free seamless DNA cloning methods have recently become available.

Plasmids were created utilising standard DNA cloning methods.

Using standard DNA cloning methods, the regulatory region carrying functional variants can be cloned upstream of a reporter gene, such as firefly luciferase (40).

Advances in modern sequencing technologies, bioinformatics analysis tools, and environmental DNA cloning methods should allow for a movement in natural products discovery away from a random microbe screening approach to a more systematic sequence-based screening approach that will allow for the functional examination of gene clusters that remain hidden in the environment.

We have developed a novel DNA cloning method named ABI-REC.

Seamless Ligation Cloning Extract (SLiCE), which was initially developed as a novel bacterial cell extract-based DNA cloning method, utilizes the in vitro homologous recombination activity of Escherichia coli cell extracts.

Here, we present both the promising and problematic nature of soil metagenomics and discuss the factors that should be considered when selecting soil sampling, DNA extraction, purification, and cloning methods to implement based on the ultimate study objectives.

Despite the large diversity of existing DNA shuffling protocols, standard cloning methods based on restriction enzymes are not widely used in these protocols.

The successful application of a soil metagenomic approach depends on selecting the appropriate DNA extraction, purification, and if necessary, cloning methods for the intended downstream analyses.

Employing standard soil metagenome cloning methods, a saturating cosmid library was constructed using DNA extracted from DFD0097 soil10.

Two highly engineered cloning methods of mating-assisted genetically integrated cloning (MAGIC) in vivo [7], together with sequence and ligation independent cloning (SLIC) in vitro [8], have all been developed to facilitate the construction of recombinant DNA molecules.