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Such as, dioscin is a TOP1 inhibitor, inhibits relegation and stabilizes the DNA-TOP1 complex in the cleaved DNA form, ultimately leading to breaks of DNA chains and cell death.
A linker molecule is required for the attachment of biorecognition molecules (e.g., antibodies, enzymes, DNA chains, and aptamers) close to the transducer surface.
A theoretical description is presented in terms of the free energy of mixing DNA, PEG, and solvent, the elastic part of the free energy for DNA chains, and the translational entropy of the low molecular ions.
Hg2+ aptamers were immobilized on magnetic nanoparticles (NPs) and HCR process happened without Hg2+, forming long DNA chains and allowing fluorescent indicator (SYBR Green) intercalated in the double helix.
It is generally believed that the organic solvent screens the electrostatic repulsion between DNA chains and condenses DNA mainly by excluded volume interactions [16], [17], [18].
It, therefore, seems likely that during FtsK dsDNA translocation, the catalytic mechanism uses just one of the two DNA chains and that translocation is 5′ 3′ with respect to this chain.
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DNA methylation occurs on the C5 position (the 5th carbon of the cytosine pyrimidine ring) of CpG dinucleotides along the DNA chain, and forms one of the epigenetic mechanisms controlling and modulating gene expression.
It removes a section of the defective DNA chain and fills it based on a complementary chain [ 1].
An efficient search for the correct DNA sequence is achieved because NikR uses both a nonspecific, low-affinity, conformation to move along the DNA chain and a specific, high-affinity, conformation to recognize and bind to the operon.
Double-isotope labelling in conjunction with hydroxyapatite chromatography allowed the evaluation of drug effects on initiation of DNA replicons, DNA chain elongation and DNA damage (single-strand breaks), and their correlation with eventual cell viability.
For comparative purposes, we tested the two models of PNImodeler (DPI1 and DPI2) and PROMO on DNA sequences of recent TF- DNA complexes which were deposited into PDB after December 2013 (DNA chains D and E of 3WTV, DNA chains C and D of 4CHU, and DNA chains E and F of 4ON0).
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