Sentence examples for dna buffer from inspiring English sources

PCR reaction was performed in a final volume of 15 μl containing 5 pM/sample of primers, 0.25 mM/sample dNTPs, DNA buffer 1X/sample, 1U/sample Taq polymerase and 50 ng of genomic DNA.

To amplify DNA using PCR, the following are required: two 3' primers for the strands of DNA, DNA polymerase to synthesize strands of DNA, buffer solutions, and cations, and deoxynucleotide triphosphate.

Total genomic DNA was isolated using the QIAGEN Genomic-tip 100/G and Genomic DNA Buffer Set (www.1.qiagen.com) with extended cell lysis (1 h) and two additional washes of the precipitated DNA.

Genomic DNA from boar N and the reference animal was isolated from lung/liver tissue by the use of Genomic-tip 100/G, Genomic DNA Buffer Set and Genomic DNA Handbook from Qiagen.

A small fragment (ca. 1 cm) of each coral was sampled, placed directly in Chaos DNA buffer (a concentrated guanidine thiocynate buffer), and the DNA later extracted using published protocols [67].

poly (dI dC) (Pharmacia) 1.0 ug/ul), 60 ug Nuclear Extract, 0.05 ug bead DNA, Buffer D to 50 ul on ice in a 96 well round-bottom polypropylene plate (Costar).

A few years ago, there was great excitement about telomeres, Liz Blackburn's specialty — DNA buffers that protect the ends of chromosomes just as plastic tips protect the ends of shoelaces.

Total genomic DNA was purified from 30 ml YPD culture using Qiagen Genomic-Tips 100/G and Genomic DNA Buffers as per the manufacturer's instructions.

Genomic DNA was isolated from a pure culture of SG61-1L with Qiagen Genomic DNA buffers and 500/G genomic tips (Qiagen, Hilden, Germany).

Samples were pooled by phenotype (resistant and sensitive pools) and genomic DNA was extracted from 30 mL of YPD cultures using QIAGEN Genomic-Tips 100/G and Genomic DNA Buffers as per the manufacturer's instructions.

Other buffers: DNA loading buffer (6×) was from Novagen; SDS Sample buffer (4×), 250 mM Tris-HCL pH 6.8, 40% glycerol, 8% SDS, 0.004% Bromophenol Blue, 20% Mercaptoethanol, dissolved in milliQ water and frozen at −20°C.