Exact(1)
Six wheats (Table 2) were tested separately in a genomic DNA background of seven different species (soy, maize, rye, barley, oat, canola, sugar beet), giving a total of eight different DNA sources per sample, all at levels of 12.5% per ingredient.
Similar(7)
We used the following parameters: method = EM2, threshold = 0.875, and the random DNA background with equal proportion of the four nucleotides.
The novelty of this marker with respect to other existing compound genetic markers (SNP STR for example) is the use of extended sequence polymorphisms (3 15 bp of the DIP), which allow the sensitive and specific amplification of the minor DNA contributor in the presence of large quantities of foreign DNA background.
Raw Cp values were efficiency corrected, and any signal of genomic DNA background was removed using GENEX (http://www.multid.se).se
Input, sequencing of input genomic DNA (background control).
For the amplified immunoprecipitated DNA, the background of non-specific IgG immunoprecipitation was subtracted from the calculated ratio between the data derived from the histone-specific immunprecipitation and input DNA.
If we accept the published value of 6⅔ picograms (pg) of DNA per cell (8), then Byrne et al. (7) were able to detect 6 copies of male DNA in a background of DNA from 6000 cells.
This was also supported by Balic et al. [ 30] who showed that analyzing DNA methylation 1% methylated DNA in the background of unmethylated DNA could be reproducibly detected in fresh frozen as well as in FFPE samples.
Related(1)
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