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Because of assay failure and limited amounts of DNA available from each subject, DNA methylation analysis was successful on varying numbers of subjects for each sequence.
In only one of the four Upper East Side cases was a sample of the assailant's DNA available, the law enforcement official said.
The one sample of the assailant's DNA available to investigators did not match, however, and investigators found Mr. White's alibis to be sound.
"We only had a minute amount of DNA available to work with, just 40 billionths of a gram.
Dr Bowler continued: "The amount of data we have released is already enormous; it is one of the largest databases of DNA available to the scientific community.
The final sample of individuals with DNA available included 321 cases and 653 controls.
Given the size of eukaryotic genomes, the amount of DNA available for non-specific interactions is enormous with respect to the specific binding sites for a particular factor.
However, we were unable to perform an array-based comparative genomic hybridization on this case as there was no high quality genomic DNA available.
Nested PCR was performed to enhance the efficiency of the amplification in order to increase the amount of DNA available for cloning.
Cases included in the GWAS study were white non-Hispanic SCA cases with DNA available (a blood or tissue sample was available in 59% of cases).
The percentage of cells in the S phase served as a good predictor of the amount of nascent strand DNA available in the preparation (see Supporting Tables S1).
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