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Our results show that both domains alter the DNA architecture from the relaxed morphology observed in control DNA samples to compact circular complexes, meshes, and networks, offering important insights into the multivalent character of the 11 ZF CTCF domain.
Local DNA architecture can prescribe enhancesome membership.
G-quadruplexes and Z-DNA are two important non-B forms of DNA architecture.
Thus, growth factor regulation of ribosomal transcription likely acts by a cyclic modulation of DNA architecture.
TRF1 is a homodimer with roles governing DNA architecture and negatively regulating telomere length.
The effect of DNA architecture on reconstituted nucleosomal arrays is examined for both linear and fully relaxed circular DNA (ring DNA).
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We also discuss some recent data on interlocked DNA architectures such as entirely double-stranded DNA rotaxanes.
We illustrate both the use of simple DNA artworks for sensing, computation, drug delivery and the application of more complex DNA architectures as scaffolds for the construction of protein and nanoparticle arrays.
To date, many three-dimensional DNA architectures of varying sizes and shapes have been precisely formed.
Due to the molecularly programmable as well as modular nature of DNA, such designer DNA architectures have great potential for in cellulo and in vivo applications.
While, individually, these proteins stabilize alternative DNA architectures consistent with either plectonemic or toroidal coiling of DNA, the combination of histone-like protein, histone-like nucleoid structuring protein, and integration host factor produces a conspicuous semiperiodic structure.
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