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It has the potential to be extended to other isothermal single-stranded DNA amplification techniques.
The latter criterion, although conservative, was the focus of one of the most successful recent approaches to detect viable cells in combination with DNA amplification techniques.
DNA amplification techniques are being increasingly applied to detect, identify, and quantify mycotoxigenic fungi in foodstuffs, but the inability of these methods to distinguish between viable and nonviable cells might lead to an overestimation of mycotoxin-producing living cells.
A total of 659 specimens (601 respiratory specimens and 58 nonrespiratory specimens) were collected for evaluation using three DNA amplification techniques: newly designed bPCR-ELISA, in-house single-tube nested PCR for IS6110 gene sequence (nPCR), and commercial automated assays, the Cobas Amplicor System from Roche Diagnostic Systems (aPCR).
The detected RNA transcripts were used to detect viable cells in combination with DNA amplification techniques.
Rapid DNA amplification techniques allow timely diagnosis; unfortunately, these techniques are often not available in the poor-resource setting.
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The new test uses a DNA amplification technique called polymerase chain reaction or PCR to test for tick-borne pathogens.
Polymerase chain reaction (PCR) is a popular DNA amplification technique and can create millions of amplicons of a target sequence in a short period of time1,2,3,4.
Rolling circle amplification (RCA) is a sensitive, specific and reproducible isothermal DNA amplification technique for rapid molecular identification of microorganisms.
We described previously a novel DNA amplification technique, termed ramification amplification (RAM) (Zhang et al., Gene 211 (1998) 277).
As an isothermal DNA amplification technique, rolling circular amplification (RCA) has been demonstrated to be a versatile tool in many fields.
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