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However, the DNA amounts of pBMB7921 (512 kb) and pBMB8240 11488 kb), which are similar in size, were quite different.
We found that the DNA amounts of the plasmids ranged from 355 kb to 1443 kb (Table 1).
Usually, Q-PCR assays are reported to yield Cts <29 for DNA amounts of 10 ng or less.
Equal DNA amounts of four samples with different sample specific barcode sequences were pooled to a final concentration of 10 ng/µl.
Among them, the DNA amounts of pBMB8513, pBMB95, and pBMB400, whose sizes are very different, were quite close to each other (636 kb, 612 kb, and 577 kb, respectively).
The absolute DNA amounts of the samples were calculated based on the values of the G1 peak means.
Similar(43)
These findings provide insight into both the total plasmid DNA amount of B. thuringiensis and the strong ability of the species to harbor plasmids.
Nevertheless, in the latter group, 44% of the samples (n = 45) also had a DNA amount of lower than 295 ng.
It is reasonable to suggest that the total plasmid DNA amount of B. burgdorferi B31 is more than that of its chromosome, also.
These data showed that the total DNA amount of the 11 plasmids was equivalent to 8746 kb at the mid-exponential growing stage (OD600 = 2.0) (Table 1).
The 1 C DNA amount of P. obtusifolium was 4.94 ± 0.039 pg (Table 2).
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