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Relative TYLCV DNA amounts were normalized against T1 tomato.
Figure 3: Relationship between different TYLCV DNA amounts and TYLCV susceptibility.
By using quantitative PCR, DNA amounts as low as 100 fg of R. solani AG-2-1 were detected.
The detection of putative hybrids based on variation in relative DNA amounts was problematic due to the occurrence of aneuploidy.
In total, 84 offspring showed relative DNA amounts deviating from tetraploid B. rapa, four of which were hexaploids.
Samples selected for ≤75 bp exhibited 99-100% reportable SNPs across varied DNA amounts and as low as 0.5 ng.
Low TYLCV DNA amounts shown in TYLCV-tolerant tomato are thought to be the result of siRNA production and DNA methylation targeted to viral sequences35.
(D) Relative DNA amounts of TYLCV in TYLCV-susceptible and -tolerant tomato plants germinated from seeds collected from TYLCV-infected tomatoes.
Thus, significantly higher DNA amounts are expected in autumn as a consequence of the higher concentrations of veligers in water samples.
According to the amount of sample DNA, TruePrep DNA Library Prep Kit V2 for Illumina of different initial DNA amounts (50 ng for TD501, 5 ng for TD502, 1 ng for TD503, Vazyme Biotech, Nanjing, China) were chosen.
The DNA amounts and full sequencing details can be found in Supplementary Table 4. Replication timing WA scores were calculated according to Hansen et al.6 with slight modifications.
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