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The resulting embryo carries DNA from its parents as usual, but its mitochondrial DNA – amounting to 0.2% of the total – comes from the donor.
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The optimum DNA amount was 100 pg to 10 ng.
DNA amount in cells is often the single parameter measured for cell cycle studies by flow cytometry.
Comparatively to BP1 method, BP2 method yielded low DNA amount with a little contamination by the respective substances.
By using quantitative PCR, DNA amounts as low as 100 fg of R. solani AG-2-1 were detected.
The detection of putative hybrids based on variation in relative DNA amounts was problematic due to the occurrence of aneuploidy.
In total, 84 offspring showed relative DNA amounts deviating from tetraploid B. rapa, four of which were hexaploids.
Samples selected for ≤75 bp exhibited 99-100% reportable SNPs across varied DNA amounts and as low as 0.5 ng.
An increase in DNA amount did not significantly increase the number of transfected cells but clearly reduced cell recovery.
It was observed that β-CD was playing a key role in improving the adamantane-labeled DNA amount modified on the electrode surface.
In order to obtain a linear relationship between cellular fluorescence intensity and DNA amount, analyses are performed with fluorescent molecules that bind specifically and stoichiometrically to DNA.
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