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The DNA amount was quantified with Hoechst Dye 33258.
The DNA amount was quantified as percent of the amount measured in the input sample using the X0-method [ 78].
Endogenous DNA amount was quantified by a primers/probe set against the human telomerase gene (Telo sense: 5′-GGCACACGTGGCTTTTCG-3′; Telo antisense: 5′-GGTGAACCTCGTAAGTTTATGCAA-3′; Telo probe: VIC 5′-TCAGGACGTCGAGTGGACACGGTG-3′ TAMRA).
Endogenous DNA amount was quantified by a primer/probe set against the murine β-actin gene (β-Act sense: 5′-AGAGGGAAATCGTGCGTGAC-3′; β-Act antisense: 5′-CAATAGTGATGACCTGGCCGT-3′; β-Act probe: VIC 5′-CACTGCCGCATCCTCTTCCTCCC-3′).
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% RNA DNA hybrid amounts were quantified using quantitative PCRs on DNA samples from DIP and total DNA with the DyNAmo HS SYBR Green qPCR kit (Thermo Scientific, Waltham, MA).
Relative protein amounts were quantified using ImageJ.
At native condition and after decellularization the total amount of DNA was quantified using a DNA isolation kit for tissues (Qiagen, Venlo, The Netherlands).
The amount of total DNA was quantified by UV spectrophotometry.
Briefly, after nucleic acid extraction, the total amount of DNA was quantified.
All cleaned PCR products were run on a gel to estimate the amount of product, and in addition, the amount of DNA was quantified with an ND-1000 spectrophotometer (Nanodrop Technologies).
The amount of DNA was quantified using a Qubit fluorometer (Invitrogen) following the manufacturer's instructions.
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