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Importantly, addition of a 5-fold excess of cells from the DLN of CD200KO or CD200R1KO populations did not alter the measured frequency of cloneable tumor cells from DLN of control mice at any of the time points investigated (ns = not significantly different).
Compared to control rat IgG, anti-4-1BB increased the number of endogenous CD4 and CD8 T cells in the spleen and DLN of the mice.
Similarly, the number of total CD8+ T cells at day 2 was significantly increased in the dLN of stg mice (Fig. 2E).
However, the total accumulation of the responding CD8+ T cells was substantially higher in the DLN of influenza infected animals (> 3 fold) than in the DLN of vaccinia infected animals within the first 24 hrs following the onset of T cell division.
The results reported above, together with the recent report by Rudensky and collaborators [21], prompted us to determine the chemokine levels in the dLN of stg and dtg mice upon antigen-specific immunization.
To verify whether the suppressive effect of MSCs was exclusively dependent on the induction of Th2 lymphocytes, we looked for the induction of regulatory cells in spleen and DLN of injected mice.
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Co-immunization with APLs did neither affect the population of regulatory T cells nor the expression of CD25, and stimulation of DLN cells of co-immunized mice with APLs in vitro did not induce the expansion of regulatory T cells (data not shown).
The absolute number of antigen-loaded DCs recovered at day 1 from the dLNs of dtg mice was significantly lower compared to that recovered from the stg ones.
Specific loss of tERK-pulsed CFSE+ targets occurred only in the spleens and dLNs of mice that received DUC18 T cells (Figure 3A).
GCs in the dLNs of FI-MV-immunized mice peaked approximately 7 days later than GCs in the lymph nodes of VEE/SIN-H and SRBC immunized mice.
On day 7, few PNA+ CD19+ B cells were present in the dLNs of FI-MV immunized mice (0.96%), similar to the PBS control (0.14%), while the percentage oFI-MV immunized the dLNs of VEE/SIN-H (5.6%) was simicer to SRBC-immunized mice (6.16%).
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