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Subconfluent GFP+ subpopulations of SK-LMS-1_shKAISO cells underwent 1.56 cell divisions in 24 h, whereas 1.20 divisions were calculated for GFP cells.
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Cells were counted every 48 h and the number of cell divisions was calculated (left panel).
The number of cell divisions was calculated based on the assumption that the dye of the mother cells would be equally divided into both daughter cells, resulting in halving of fluorescence intensity.
The number of cell divisions was calculated using the following formula: After fractionated radiation, cells were stained according to Darzynkiewicz et al. [ 19].
The percentage of parallel divisions was calculated for different animals and weighted mean and variance for the percentages for each age was determined.
Five fields (at least 100 cells of each field) were randomly selected and the percentage of cell division was calculated.
The likelihood of such a division is calculated according to model Mobs for C1, C2, C3 and IG sites.
Additionally, the proportion of segmentally duplicated FAexpTRL in relation to the total number of FAexpTRL per 10% pseudomolecule division was calculated.
Proliferation was measured by flow-cytometric analysis of CFSE dilution, and division indices were calculated by computing the weighted fractions of all the divisions as per (D'Alise et al., 2008).
Divisions into levels were calculated so that the number of subjects in each was equal.
For each of the strains the indices of cell division time asymmetry were calculated after selection for an estimated 1000 and 2000 generations.
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