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The number of cell divisions was calculated using the following formula: After fractionated radiation, cells were stained according to Darzynkiewicz et al. [ 19].
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The proliferation of WT responder T cells were assessed on d 3 by flow cytometry; division index was calculated using the FlowJo software (see Additional file 1: Figure S2D for more detail).
The normalized mean number of division that cells have undergone was calculated using method of De Boer and Perelson, where Xn(t) = the number of cells undergone n divisions by time t [51].
MAGGIC score was calculated using online calculator.
The division over the months of this annual inflow was calculated using the Pardé Coefficients from Table 5.2 of the Hydrological Atlas [51].
Gene family age (the oldest phylogenetic division in which a gene from the same family is found) was calculated using Ensembl Compara phylogenetic trees (33).
MBL was calculated using the equation (1).
The junction potential was calculated using pCLAMP (version 10.7.0.).
An annual rate was calculated using working-age Americans.
Statistical significance was calculated using Student's t test.
Statistical significance was calculated using the impaired t-test.
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