Elizabeth Cowles, 38, who is known as Betsy, oversees the company's broadcast division, video and film production, insurance and real estate in downtown Spokane.
Time-lapse fluorescence microscopy analysis of GFP-expressing EC in co-culture revealed no apparent cell division (Video S1).
Information across the entire division video is combined by calculating the pixel-by-pixel standard deviation image.
In meg-3 zygotes, oocyte granule disassembly was delayed but was eventually completed before the first division (Video 3).
Next, motion contrast enhancement was applied, which involves calculation of a division video and a standard deviation image.
In the controls, cells became smaller and the number of cells doubled after each division (Video 1).
During mitosis, a few new granules appeared in the posterior cytoplasm, but these remained small (<1 micron) and most were not maintained during the division (Video 2).
The division video eliminates static portions of each frame (e.g. photoreceptors), while emphasizing regions of high relative motion (e.g. moving parcels of flow due to individual blood cells).
Briefly, for each vessel of interest, the vessel centerline is extracted from an image of perfused vessels [ 20], and then intensities along the extracted centerline are read off from the division video.
Shortly after ovulation, P granules that were present in the oocyte cytoplasm (oocyte granules) disassemble and new granules (zygote granules) form throughout the cytoplasm as the maternal pronucleus completes the second meiotic division (Video 1, Figure 1A, Figure 1 figure supplement 1).
