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Sometimes the original division of samples into groups is not always reflected in large differences in the corresponding spectra.
However, division of samples is possible without much difficulty, allowing for example, mechanical testing to be performed on one half and histological staining on the other.
Column 1 shows the division of samples into three groups according to percentage invasive tumour content.
This procedure enabled division of samples into classes with high and low expression of microRNA.
The division of samples to negative or positive as regards to nuclear staining was assessed according to mean value.
This procedure enabled division of samples into two levels with high and low expression of miRNA (over and under the cutoff point, respectively).
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Given M modulator genes of interest, expression profiles of them are extracted from the microarray dataset and z-transformed (subtraction of sample mean followed by division of sample standard deviation) across samples so that each modulator approximately follows standard normal distribution.
Published aDNA protocols were used at McMaster including the division of sample preparation, extraction and PCR setup rooms; the use of protective clothing; and techniques to minimize the contamination risk including UV light exposure, bleach sterilization, use of PCR workstations, and filtered pipette tips [ 32].
The results showed the division of the samples into groups that were probably due to the presence of different mortar types manufactured in different periods.
The division of the samples into groups describing geographical regions and landscape type enabled evaluation of the spatial trends in contamination of soils with PAH compounds.
Specific reasons for failing to detect mixed infections would include low initial numbers of minority strain cells in sputum, stochastic growth in culture and the physical division of initial samples into parts (typically only one of which is genotyped).
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