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Due to the reported functional, morphologic, and neuroanatomical heterogeneity of OPC47, and its potential influence on cell division mode, cell cycle lengths, and differentiation1,9,48,49, we focused our studies on OPC in the corpus callosum.
Division mode: spots continue to multiply by binary fission after their elongation (Fig. 2D and Movie S3).
(ii) The multiplication pattern generates multiple CZ spots by the division mode (Fig. 3C and Movie S6) or by the emergence mode (Fig. 3D and Movie S7).
Electrode signals were digitised together with the signals of a technical bat detector (D100, Petterson Inc. Sweden) operating in frequency division mode using an AD converter (Powerlab, AD-Instruments Inc. Germany) connected to a laptop (Fujitsu Computers, Siemens).
These patterns fall into two classes according to their proliferation mode: (v) dichotomous pattern by the division mode (Fig. 3G and Movie S10) and (vi) monopodial pattern by the emergence mode (Fig. 3H and Movie S11).
In addition to the automatic recording of bat activity, a 30 minute transect was carried out at each sampling site using a bat detector (S-25 Ultrasound Advice, London) set to frequency division mode.
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Reinstalling active β-catenin signaling rescues division modes and ameliorates growth defects.
To assess proliferation and cell division modes in demyelinated lesions, demyelination was induced by a single LPC injection into the CC or the spinal cord of mice previously injected with tamoxifen.
To assess proliferation rates and cell division modes of OPC, mice were subsequently treated with 0.1 mg/mL EdU in drinking water for 14 days and resected brains were processed for immunofluorescence.
Our analyses of cell division modes support the hypothesis that Lgl1 restricts NG2 during ACD as part of the mechanism to turn on OL differentiation in the NG2-negative progeny.
This structure can be observed across the Apicomplexa and is independent of the related, yet different, division modes encountered across the Apicomplexa [12].
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