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To trace fertilization products derived from such incomplete gamete fusion, an Arabidopsis line that express H2B-RFP and ΔFWA-GFP as egg and central cell division marker respectively [14] [16], was produced and pollinated with +/gcs1+/GAA line pollen, in which sperm cells homozygously express GAA (Figures 5A C).
However, analysis with the previously defined asymmetric cell division marker Numb detected asymmetric segregation in a fraction of mitotic GSCs.
The gradient of expression is similar to that for the cell division marker CycB1 1::GUS [ 37].
ASCL1 and DLX2 maintained high expression in the proliferative zones and showed extensive immunofluorescent double-labeling with the cell division marker Ki-67.
The cell division marker Ki67 was positive in tumor cells and negative in histiocytes and osteoclasts; Stains for common leukocyte antigen (CD45) and for the histiocytic marker CD68 were positive in histiocytes and osteoclasts, but were negative in tumor cells.
Examination of the RAM of these mutants showed that QC size is increased as is cell division in the initial cells, as measured by the cell division marker pCYCB1 1::GUS.
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Healthy human kidneys also have dividing cells in nephron tubules based on staining for the cell division markers Ki67 and proliferating cell nuclear antigen (PCNA) [43].
Also, the expression of G2-M transition and cell division markers, as CyclinB1 1 and CDKB2 1 was analyzed [ 49].
qRT-PCR data showed that expression of Cdt1, a preRC gene repressed by ABAP1, is increased in AIP1KD; as well as the expression of other cell division markers.
Furthermore, antibody staining with the cell-division markers PCNA (proliferating-cell nuclear antigen) and Ki67 detected positive tubule cells in adult human kidneys [ 111].
These include evidence in the brain of oxidative and other free radical damage, reduced anti-oxidative capacity, loss of synapses, expression of cell division cycle markers and neurons displaying hyperploidy, to name but a few.
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