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Genotype I, which includes the vast majority of the human HAV isolates, has been divided further into subgenotypes IA and IB.
Most genotypes are now divided into subgenotypes with distinct properties.
Several genotypes are further divided into subgenotypes.
The HAV genotypes are further classified into subgenotypes with sequence variability of <7.5% [ 11].
Phylogenetic analysis of the complete S region allowed for further classification into subgenotypes.
Genotypes A-D and F have been further classified into subgenotypes [ 16] based on nucleotide divergence of 4 to 7.5 % [ 12].
Most genotypes can be split into subgenotypes that differ >4% from each other, such as HBV-A1 and HBV-A2 [ 7].
Based on viral RNA sequence, there are at least 2 viral genotypes of BVDV that can be further divided into subgenotypes [ 2, 3].
Previously, subgenotypes or clusters in different genotypes of DENV had been identified, and genotype I of DENV-1 was further classified into subgenotypes A, B, C, D, and E. To elucidate the origin and dissemination pathway of DENV in Kaohsiung, the E gene was amplified from 33 DENV isolates using real-time reverse transcription polymerase chain reaction for phylogenetic analysis.
HBV is classified into 8 genotypes, HBV/A through HBV/H, and is further divided into subgenotypes (5 ) that have a distinct geographic distribution and are associated with different disease outcomes.
Phylogenetic comparison of the genomic sequences of viruses from the three BPI3V genotypes provided evidence that two of the genotypes could be further subdivided into subgenotypes based on degree of divergence.
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