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First, we noticed that disassembly of oocyte granules was incomplete in meg-3 meg-4 zygotes (Video 2, Figure 1 figure supplement 1), causing a few oocyte granules to persist through the first mitotic division (arrows in Figure 4A).
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c The megasporocyte undergoes the first meiotic division (arrow).
c Soon, the megasporocyte undergoes the first meiotic division (arrow).
d An anticlinal division (arrow) occurring in the terminal cell results in the formation of a four-celled embryo.
d After the first meiotic division, the functional dyad at the chalazal end of the ovule successively undergoes the second meiotic division (arrow), and the non-functional dyad at the micropylar end has degenerated (arrowhead).
The neurons that enter the vertical lobe just above the γ division (arrow in Fig. 3F, also 7F, 7H2) correspond to the feedback neurons that connect mushroom body calyces with mushroom body lobes.
Myo8A-GFP localizes to the phragmoplast midzone (arrow) and the cortical division site (arrow heads).
Eight successive asymmetrical periclinal cell divisions (yellow arrows) follow the first anticlinal division and generate, successively, the epidermis endodermis, sclerenchyma layer, exodermis, and five layers of the cortex.
(H – H′ ) Schematic (H ) of a late second instar disc (as shown in F ) showing active mitotic division (Black arrow) parallel to the disc epithelium.
The same pattern as described for the radicle meristem is observed: periclinal divisions for columella initials (red arrows), anticlinal divisions for peripheral root cap initials (white arrows), and three sequential periclinal divisions occur, giving rise to epidermis, exodermis, sclerenchyma, and endodermis (yellow arrows).
Myo8A-GFP remains at the cortical division site (C, arrow heads) and is at the phragmoplast midzone (C, arrow).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com