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To compensate for the different segment lengths of each analyzed artery, all volumetric data were divided by segment length and displayed as volume index (mm3/mm) [ 31].
The sum of such intervals is then divided by segment time length (for example, 5 minutes), from which the NFR can be derived.
We assumed that segments with the least fluorescence concentration (fluorescence within the segment divided by the segment area) correspond to cell area far away from the GFP-ParB/ parS complex and therefore their fluorescence values report the concentration of freely diffusing GFP-ParB.
Assuming that the physical length of these genomic intervals is correctly predicted by the genome sequence, the map length divided by the segment size yields a recombination rate (cM/Mb) point estimate for each scaffold.
The effective centerline, which removes two marginal segments from the centerline, is divided by equal distance segments and points in-between segments are extracted.
A false positive rate was calculated as the number of expected segments divided by the number of observed segments of the same length.
Consecutively, the relationship of the cost/DDD in the remaining 10% segment and the DU90% was calculated (cost/DDD of remaining 10% segment divided by cost/DDD of DU90% segment) [ 6 ].
It seems to be typical in applications that in fact, the convex domain D is divided by a line segment into two parts, in each of which the function A described above is well defined.
Subsequently, the values calculated were divided by the total segment time and reported as snoring frequency (Sf) and breathing frequency (Bf) (snores/min and breaths/min, respectively).
Dendritic field coverage was calculated essentially as described (Parrish et al., 2009) by measuring the area covered by dendrites (polygon formed by connecting all dendritic terminals of the field) divided by the total segment area using Fiji/ImageJ (NIH, Bethesda, MD).
Except for the group of the oral blood-glucose-lowering drugs (ratio 3.6), the groups had mean ratios between 0.9 and 1.8 for the costs/DDD in the remaining segment divided by the costs/DDD for the DU90% segment.
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