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As a result, their contrasts between low and high prey genetic diversity were performed with a single genotype (CBS strain).
However, these studies were restricted to the basic phylogenetic level and no phylogeographic studies including estimates of haplotype and nucleotide diversity were performed.
Analyses of nucleotide and haplotype diversity were performed separately for each population as well as for all populations grouped together using the software DnaSP v5.10.
Estimates of founder colony diversity were performed on mosquito samples 3 5 months prior to mapping by pooled sequencing, thus diversity and divergence as shown in Fig. 1 should accurately represent diversity present at the mapping stage.
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Alpha diversity was performed using the Shannon index, Chao 1, Observed species index and Simpson index.
In vitro selection of functional SH3 sequences that can bind to the ligand peptide VSL12 [21] from each library (containing 3×1013 molecules with up to 0.6 2.4×1011 diversity) was performed by mRNA display (Fig. 3).
Characterization of the library (e.g., number of members, diversity) was performed as described elsewhere [ 11].
Investigation of spa type diversity was performed using a high-resolution melting (HRM) assay of the spa locus [ 24].
First, it is important to note that our contrast between low and high prey genetic diversity was performed with a single genotype (CBS) for the low diversity treatment.
The Sliding-Window analysis of nucleotide diversity was performed with window size set at 100 bp and steps of 25 bp.
The calculation of HIS and DIS may seem to contrast the SS calculation in a single trial where no adjustment for heterogeneity or diversity is performed.
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