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The observed diversion of flux to lactate is mainly due to the necessity of NAD+ regeneration (Yang et al. 1999a).
A diversion of flux from these reactions towards L-malate production could result in depletion of these amino acids.
In both cases there is a metabolic flux diversion towards product formation.
Further diversion of carbon flux into neutral lipid droplet formation was investigated by the overexpression of DGA1 or ARE1 and by the co-overexpression of a compatible lipase, TGL1, TGL3 or TGL5.
In this review we outline the array of metabolic engineering strategies employed for the efficient diversion of carbon flux from native biosynthetic pathways to the overproduction of a target alcohol.
In this study, we demonstrate production of C-4 carboxylic acids non-native to the host, specifically crotonic and butyric acids, from methane in an engineered M. buryatense 5GB1C by diversion of carbon flux through the acetyl-CoA node of central 'sugar' linked metabolic pathways using reverse β-oxidation pathway genes.
This study presents evidence for a carbon flux diversion from the glycolytic pathway to the pentose phosphate pathway as a consequence of plasmid maintenance.
The knockout of fabF results in the flux diversion into other reactions carried out by FabB.
A model for carbon flux diversion towards fructan biosynthesis in perennial ryegrass is shown in Fig. 6.
These four groups are FTs, FEHs, invertases, and other genes potentially involved in carbon flux diversion towards fructan biosynthesis (Additional file 15).
We predicted a diversion of metabolic flux from ethanol to acetate in more oxidizing environments, which we experimentally confirmed by growing ICGEB2008 on nitrate.
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