The research interest of Dr. Scholz and his group directly corroborates with the main objectives of the REP-LECOTOX project, namely, the trainings organised there directly helped the introduction of ecotoxicogenomics into laboratory practice of LECOTOX and diversification of in vitro methods on widely recognised but also new promising biological models.
The clearly observed pattern of considerable phage-mediated diversification in highly related L. monocytogenes isolates with nearly identical genome backbone sequences also demonstrates that the absence of any possibility for phage-mediated diversification during in vitro evolution studies of bacteria represents a major constraint associated with these studies.
Novel, simplified analogues of the microtubule-stabilizing anticancer agent laulimalide, including the first derivatives with unnatural side chains, were designed by molecular modelling, synthesized by a late-stage diversification strategy, and evaluated in vitro for growth inhibition of human ovarian carcinoma cell lines (A2780, A2780/AD10).
Novel analogues of the microtubule-stabilising agent dictyostatin were designed using existing SAR information from the structurally related discodermolide, synthesised by a late-stage diversification strategy and evaluated in vitro for growth inhibition against a range of human cancer cell lines, including those known to exhibit Taxol-resistance (AsPC-1, DLD-1, PANC-1, NCI/ADR).
Discovery and optimization of antibodies that bind antigen using in vitro techniques require diversification of one or more of these CDRs.
This current opinion review will focus on recent advances made in performing in vitro pathway-driven natural product diversification and opportunities for exploiting this approach for elucidating and entering this new chemical biology space.
That in-vitro attempt did not result in a pregnancy.
He was the first to show that human oocytes could undergo in-vitro maturation, as well as fertilisation in-vitro.
"I did in-vitro and paid someone for her eggs!
The cost of each in-vitro cycle: $11,000.
It uses in-vitro testing and culture testing.
