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Two genetic sources were donors of newly emerged bees: i) genetically diverse wild type stocks from four colonies headed by openly mated queens of Californian commercial origin, and ii) a standard research stock maintained by instrumental insemination, using five colonies headed by queens inseminated with 1 2 drones (males) each.
10.7554/eLife.08146.006 Figure 2. Function of diverse wild type (WT) and loop 3 mutant intergenic region (IGR) internal ribosome entry site (IRESs) in rabbit reticulocyte lysate (RRL).
We utilized four genetically diverse wild type stock colonies (Apis mellifera) headed by queens of Californian commercial origin that were mated with multiple males.
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Diverse wild-type rotavirus types circulated during the study period including large numbers of G2, G8 and G12 types, with different distribution per country.
This discovery was possible because we were able to examine many genetically diverse wild-type fly lines under conditions where factors known to affect the probability of death following TBI, that is, the force, number, and timing of primary injuries, age at the time of the primary injury, and environmental conditions, were kept constant.
To address the molecular mechanism of Hox gene silencing in dependence of Lsh and DNA methylation we examined nucleosomal positioning, RNA Pol II binding, RNA splicing and diverse histone modifications comparing wild type cells to hypomethylated Lsh−/− cells, or dynamically regulating DNA methylation levels in WT and Lsh−/− cells.
We evaluate the method by examining protein synthesis rates in diverse cell types of live, wild type or mRNA translation-defective Caenorhabditis elegans animals.
Amongst non-human animals, experimentally-provided raw materials have been shown to induce structural differences (compared to wild type) in diverse artefacts, for example, in the protective cases of caddis fly larvae (Trichoptera), when produced from un-preferred raw materials [ 40], or in the nests of zebra finches (Taeniopygia guttata), when built from floppy pieces of string [ 41].
DNA from 92 wild type chickens from diverse traditional breeds did not carry the c.535A > T mutation (Table 2).
Despite the involvement of EWSR1 in these diverse sarcomas, the in vivo function of wild type EWSR1 remains unclear.
Therefore, the conformational isomerism and additional flexibility observed in wild type PFN1 may be necessary for its diverse roles.
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