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The new grants explore inter-individual variation in DNA accessibility, protein levels, telomere lengths, and DNA modifications from diverse tissue samples whose collection began in 2010 across hundreds of individuals.
We carried out a large-scale survey of gene expression in porcine by establishing expression profiles for approximately 20.000 genes represented by 26.877 microarray cDNAs across 23 healthy and diverse tissue samples.
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A total of 17 Picea glauca cDNA libraries were prepared from a diverse tissues sampled to maximize the diversity of the isolated genes.
When comparing the microbial diversity between tissue samples, however, the tumor microbiome was more diverse than the normal microbiome.
Tissue array analysis was performed on 54 diverse human breast tissue samples with different histologic diagnoses (Table 1).
An endogenous amplification control was not included in the assay due to the diverse nature of tissue samples being tested, since any internal control based on the squirrel genome would have to be individually optimised for each tissue type.
The main weaknesses of the study are that a large number of patients had to be excluded from the final analysis for diverse reasons, and that many tissue samples could not be properly analyzed in The Netherlands for technical reasons, including poor tissue processing or storage problems back in Uganda.
Our collected DNAm profiles included diverse tissue types in the normal samples.
These samples included diverse tissue types and exhibited a wide range of ages from 0 to 91 years (Additional file 1: Table S1).
A recent study by miRNA expression profiling of thousands of human tissue samples revealed that diverse miRNAs constitute a complex network composed of coordinately regulated miRNA subnetworks in both normal and cancer tissues, and they are often disorganized in solid tumors and leukemias [ 28].
However, for MMP-8, -10, -12, -14 and -24 a diverse expression profile was identified in the tissue samples analyzed.
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