With 66 diverse gene probes tiled on RPM version 1 covering 20 common respiratory and 6 biothreat pathogens (14 ), it was logical to use a generic, sequence-independent PCR strategy to amplify all potentially pathogen-derived sequences in an unbiased fashion before hybridization.
This, however, is but 1 member of a new family of Haemophilus TAAs, which is unique to Hae but (except for hadA) shared among conjunctivitis isolates (12 diverse strains probed, unpub. data, the authors) and among members of the BPF clonal lineage (4 examples probed, unpub. data, the authors).
These approaches are only now being applied to P. falciparum, but, as the recent publication of the P. falciparum phosphoproteome illustrates, they will provide important insight that supports the generation of diverse hypotheses probing key aspects of parasite biology (Treeck et al., 2011).
1 In this approach, an abiotic chemical functionality (reporter) is incorporated into a target biomolecule and can then react with a complementary bioorthogonal functional group linked to one of a diverse set of probes.
This offers an intriguing explanation for the observed bioactivity of acivicin and offers an opportunity to study the activity of selected enzymes using the diverse set of probes presented here.
The need is great for a diverse set of probes capable of having their fluorescence signal turned on by analytes in complex environments, so as to allow for analyte detection, quantification, and imaging.
Much of the software is based on open-source Hadoop, a framework for handling diverse data and probing it with distributed, parallel-processing computing clusters.
This difference implies that the BOLD signal and measurements of MEPs probe diverse physiological mechanisms.
