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Experimental validation of the classifier reveals it to have learned membrane activity as a unifying signature of antimicrobial peptides with diverse modes of action.
Firstly, we can see that compounds annotated as histone deacetylase (HDAC) inhibitors, which are clustered in the north-east of the Fig. 6, appear to be the most likely compounds in the dataset to be synergistic, and specifically with the compounds in the center (these are annotated with diverse modes of action, but often were kinase or phosphatase inhibitors).
With this in mind, and using fluorescence microscopy and MMP-disrupters with diverse modes of action, we have previously tested the ability of various mitochondrial probes to monitor human sperm MMP [41].
These results encouraged us to test the same strategy for drugs with diverse modes of action.
These AMPs usually have diverse modes of action so that they complement rather than replicate each other.
Independent action (also called "response addition") is used for combinations of agents with diverse modes of action.
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This finding is in accordance with toxicity experiments conducted in algae with mixtures of herbicides from one homogenous mode-of-action group such as chloroacetanilide [30] or phenylurea [31] and from diverse mode-of-action groups [32].
To the best of our knowledge, this is the first study demonstrating the diverse mode of action of celecoxib on human breast cancer cells, which may be dependent upon the cells' invasive properties and levels of COX-2 expression.
Because pfmdr1 influences parasite responses to drugs with diverse structures and various modes of action, a non-specific mechanism may be invoked through changes in FV physiology.
For example, silver nanoparticles (AgNPs) display diverse modes of inhibitory action against various pathogens such as fungi and bacterial species (Yamanaka et∼al. 2005; Lamsal et∼al. 2011).
Ancient invertebrate-type and classical insect-type defensins (AITDs and CITDs) are two groups of evolutionarily related antimicrobial peptides (AMPs) that adopt a conserved cysteine-stabilized α-helical and β-sheet (CSαβ) fold with a different amino-terminal loop (n-loop) size and diverse modes of antibacterial action.
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