One innovation of this study is that we investigated the stability of miRNAs in diverse human cell types, which allow us to not only investigate the diversity of miRNA stability in individual cells but also explore the dynamics of miRNA stability across different cellular context.
Varley KE, Gertz J, Bowling KM, Parker SL, Reddy TE, Pauli-Behn F et al. Dynamic DNA methylation across diverse human cell lines and tissues.
The group will take an epigenomics approach that they previously used to study the regulatory circuitry of diverse human cell lines and tissues, and apply it to study how these elements vary across individuals.
These results indicate that PHD3 promoter methylation is utilized by malignancies derived from diverse human cell types.
Our studies expand the expression of spastin to human fibroblasts, hiPSCs and astrocytes and indicate that the M87 isoforms of spastin are expressed in diverse human cell types.
Using Affymetrix oligonucleotide array analysis we identified common and disparate host cell responses triggered by T. cruzi infection of phenotypically diverse human cell types.
The peptides are cell-permeable against diverse human cell lines, are highly isoform-selective for PKA-RII, and can effectively inhibit interactions between AKAPs and PKA-RII in intact cells.
Three highly diverse human cell lines (HeLa, MDA-MB-231, and PC-3 cells) were treated with 5 μM 5 6 -carboxyfluorescein-labeled peptides for 6 h before washing and fixation.
It was initially characterized in diverse human cell lines, where COX activity was found to be higher than that required to support endogenous respiration rates, allowing this way some adaptability to higher respiratory needs (Villani et al, 1998).
