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One innovation of this study is that we investigated the stability of miRNAs in diverse human cell types, which allow us to not only investigate the diversity of miRNA stability in individual cells but also explore the dynamics of miRNA stability across different cellular context.
These results indicate that PHD3 promoter methylation is utilized by malignancies derived from diverse human cell types.
Our studies expand the expression of spastin to human fibroblasts, hiPSCs and astrocytes and indicate that the M87 isoforms of spastin are expressed in diverse human cell types.
Using Affymetrix oligonucleotide array analysis we identified common and disparate host cell responses triggered by T. cruzi infection of phenotypically diverse human cell types.
Three highly diverse human cell lines (HeLa, MDA-MB-231, and PC-3 cells) were treated with 5 μM 5 6 -carboxyfluorescein-labeled peptides for 6 h before washing and fixation.
It was initially characterized in diverse human cell lines, where COX activity was found to be higher than that required to support endogenous respiration rates, allowing this way some adaptability to higher respiratory needs (Villani et al, 1998).
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High-LET radiations have been shown to induce autophagy in diverse human cells.
Therefore, ETS2 has diverse activities across various human cell types; these activities are likely to be cell context-dependent involving different target genes (61, 62).
To demonstrate the application of this platform to human cells, we profiled seven diverse human cancer cell lines in four microplates (termed PM-Ms) containing 367 substrate nutrients (Figure 1; see Table S1 for a complete map of the nutrients).
The National Cancer Institute has used a panel of 60 diverse human cancer cell lines (NCI 60 cell line) (http://genome-www.stanford.edu/nci60/index.shtml) for drug-related research [ 8].
Cytotoxic effects of epothilones with diverse human cancer cell line including lung cancer cell line (NCI-H460) (Kim et al. [2003]), ovarian cancer cell line (SKOV-3) (Rogalska et al. [2013]), breast cancer cell lines (Cheng et al. [2008]), etc. have been reported in dose dependent manner.
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