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TRFLP is a community fingerprinting method in which diverse gene sequences are amplified by PCR using a fluorescently labeled primer.
Millions of sequence comparisons are required to find the most conserved regions within gene families and subfamilies, to ensure that probes are selected to span diverse gene sequences that encode similar functions.
While there are a plethora of potential pneumococcal targets for future arrays, the inclusion of MLST gene sequences in addition to diverse gene sequences would allow the strain to be simultaneously subtyped by MLST while characterizing clinically-important and biologically-relevant genes.
Candidate genes were selected for RNAi analysis based on their cosegregation with disease resistance phenotypes and representation of as many diverse gene sequences as possible.
The goal of this research was to extend this demonstration to diverse gene sequences by testing recombinant gas vesicles displaying peptides encoded by different SIV genes (SIV tat, rev or nef ).
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These results suggest that circulating strains of subtype A/H3N2 harbor diverse NS gene sequences, variations that presumably arise from frequent co-infections and genome segment reassortments in the field.
We have developed Sma3s, a fast, accurate and flexible annotation tool specifically designed for the annotation of large collections of sequences obtained from diverse gene libraries or coding sequence datasets.
This regression analysis suggests L. monocytogenes has a significantly diverse gene reservoir, and additional sequencing of LIII genomes is necessary to resolve the exact size and nature of the L. monocytogenes pan-genome.
We performed PCR using several pairs of degenerate oligonucleotide primers, which were designed to amplify the GBGT1 gene sequences from diverse species.
The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria.
After supplementing the array with Sanger sequencing of diverse regions, the 11 concatenated gene sequences were used in a phylogenetic analysis and compared to the MLST-defined clones to determine if this approach could further discriminate between genetically related strains.
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