Divergent orientation of tabular layers relates to changes in energy of currents and waves and involves time gaps and erosion.
A design modification of stand-alone cages using the divergent orientation of vertebral fixation incorporated in the box had been considered to overcome lateral bending and extension loadings [ 26].
The structural elements of a bona fide repABC plasmid [83], i.e. a canonical repABC operon encoding plasmid replication and maintenance function with its counter-transcribed small RNA in divergent orientation upstream of repC, and a palindromic 16 nt sequence GTTCTCAGCTGAGAAC fitting the par binding site consensus sequence [83] upstream of repA, were also found within the dcm region.
Our research approach can be described as a dissensus-oriented Delphi, our interest not just in exploring an overall understanding of consensus, but also in the divergent orientations and visions within the panel.
Among the 968 overlaps larger than 60 bps which we analysed, we did not find a single real one among the co-directional and divergent orientations and concluded that there had been an excessive number of misannotations.
To validate the results of the circRNA sequencing analysis, we designed primers in divergent orientation, specifically capable of amplifying the junction region of the circRNA (Fig. 3A; primer pairs listed in the Materials and Methods section) for the circRNA transcripts identified in Fig. 2. A subset of circRNAs detected by RT-qPCR analysis is shown (Fig. 3B).
Another common neighbor of rpoG is the gene for transcription elongation factor TFIIIB, with a divergent orientation in the majority of Thermoproteales and a codirectional orientation in the korarchaeote.
The low frequency of mixed TGAs (2.1%) is in correlation with the low percentage of TGAs containing at least 3 CDSs (13.9%) and of which at least two copies have a convergent or divergent orientation, this being the less frequent orientation of a CDS pair (expected frequency = 0.190 × 0.139 = 0.026).
These predicted MrpC binding sites might be involved in regulation of MXAN_2883, which lies upstream of fmgA in divergent orientation.
Co-oriented overlapping gene pairs (OGPs) represent the vast majority of these observed structures, and very few incidences of OGPs with divergent orientation have been found [ 5].
