Nucleotide sequences of the cyanase and carbonic anhydrase CCA gene cluster were sufficiently divergent from native copies that the cluster could be selectively amplified.
This observation is consistent with the expectation that recently transferred xenologous atp1 genes should be less divergent than native sequences that have resided for longer periods of time in the rapidly evolving parasitic plant genomes.
In order to detect partial CCA gene clusters, PCR primers were designed to target each gene's open reading frame (ORF) using nucleotides that were invariant in CCA gene clusters from the sequenced FOSC genomes but divergent from the native gene copies.
Genomic DNA from a diversity panel was used for re-sequencing which consisted of one individual drawn from each of the following nine divergent breeds: Awassi, Gulf Coast Native, Katahdin, Lacaune, Merino, Poll Dorset, Red Masai, Romney and Texel.
A minority of interesting genes (e.g. inhibinbetaA) displayed divergent expression preference under native and ischemic conditions with partially opposing directions of regulation in both cell types.
If mating isolation were evolving as a consequence of divergence in sexually selected traits, then males more phenotypically divergent from the female's native population should be discriminated against most strongly, regardless of predation type [5].
"I was in North Dakota, standing side by side with Native Americans," the "Divergent" actress wrote.
Significant sequence polymorphism was observed in amplified nahAc products, indicating that divergent homologs of nahAc were present in the native community.
(9) Following structure-guided mutation and selection, MmOmeRS switched amino acid specificity from the native substrate Pyl to the structurally divergent substrate Ome.
The Pch PRP8 intein and the native yeast VMA1 intein are sufficiently divergent (21% sequence identity) to preclude homologous recombination between the two during the transformation step.
