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Phylogenetic analysis of the BLAST-filtered subset revealed distinct groupings that are clearly divergent from conventional nitrogenase.
Rhilphs, Shelphs and Alphs form PPP clades, as divergent from "conventional" eukaryotic PPP phosphatases as they are from each other and from major bacterial clades.
As it was suggested by initial sequence similarity searches, Rhilphs, Shelphs and Alphs represent distinct major clades of the PPP family, as divergent from "conventional" eukaryotic and archaeal PPP phosphatases as they are from major bacterial clades.
Here we constructed phylogenetic trees with real NifH/NifD/NifK and NifH/NifD/NifK-like sequences and the phylogenetic tree revealed that NifH/NifD/NifK-like sequences are clearly divergent from conventional nitrogenase.
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