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When two or more populations were considered, the divergence time could also be derived.
Branch lengths reflect divergence time estimates based on the fossil record.
Other approaches to divergence time estimation are under active development.
We assume the divergence time to be 35 myr ago.
In addition, previous studies provided disparate divergence time estimates.
Estimating divergence time is useful to reconstruct evolutionary history.
The constraints above were used in all divergence time analyses.
The opossum-eutheria divergence time (∼155 MY) was computed as the average of the divergence time estimates in several studies [37], [38], [39] and the fly-anopheles divergence time (∼250 MY) as in [40].
The opossum-eutheria divergence time (∼155 MY) was computed as the average of divergence time estimates in several studies [27], [28], [29].
Their divergence time is estimated to be 6-7.2 million years ago, contemporaneous with the divergence time of the human and chimpanzee lineages (Figure 6C).
To ensure reliable divergence time estimates, we must start with high quality datasets.
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