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However, at least two other loci, LYSO and GF1B, also registered a strong divergence between two groups of alleles that are interpreted to have introgressed secondarily.
On the one hand, the divergence between two groups might affect the AP2 family motif length.
High relative divergence between two groups can also be the result of reduced diversity due to selection within the populations, even in the absence of gene flow [ 49, 50].
Another strength is that we used last observation carried forward in this analysis because this method is considered more conservative than general mixed model analysis, "freezing" any observed divergence between two groups by retaining the last observation made.
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At the higher levels, this classification uses node-based names to indicate points of evolutionary divergence between one group and another.
Nevertheless, we found evidences for Type-II functional divergence between three group pairs including II/III, II/IV, and III/IV, indicative of a radical shift of amino acid property [ 55].
The network revealed a distinct deep divergence (13 mutational steps) between two groups that corresponded to haplotypes [A, B], and [C, D, E, F] respectively, which was consistent with our phylogenetic tree.
The proposed method uses the Kullback-Leibler divergence (KLD) as the index of difference in distribution between two groups.
The divergence between the two groups might affect the AP2 family motif length, with a higher divergence generally being associated with a shorter motif.
If blackcaps wintering there fare worse than their counterparts in the United Kingdom or Spain, the "selection against [these birds] could promote further divergence between the two groups," Irwin says.
Finally, our analysis shows that there is relatively little mitochondrial genetic diversity within either spiculate or aspiculate homoscleromorphs, although the divergence between the two groups is substantial.
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