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‡ Sequence divergence analyzed by using the DNA distance matrix in BioEdit (http://www.mbio.ncsu.edu/BioEdit/bioedit.html).html

Actual patterns on gene expression divergence analyzed by Bedford and Hartl (2009) and by Khaitovich et al. (2006), mentioned previously, may fit to the present scenario.

Figure 2 shows the gene frequency distributions and the corresponding model fits for four representative groups of prokaryotes spanning the range of evolutionary divergence analyzed in this work.

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Plausible reasons for this divergence are analyzed in the Discussion section.

Sequences of pairs or trios of species with low divergence were analyzed by eye for the identification of diagnostic nucleotides (positions fixed within each species but different between them), which have previously proven to be robust in other species (Kerr et al., unpublished data).

Nucleotide divergence was analyzed using MEGA 5.10 software [ 36] and DnaSP 5.10.01 software [ 37].

To assess the contribution of LTR retrotransposons to this C/n divergence, we analyzed and quantified partial sequences from both LTR retrotransposon superfamilies.

We compared their genomic variation and expression divergence and analyzed overrepresented genes and their functions by using gene set enrichment analysis (GSEA; Mootha et al. 2003) program.

Synonymous and non-synonymous divergence was analyzed on a per site basis to avoid confounding by different numbers of transitions and transversions.

To further understand the mechanism of divergence, we analyzed the potential SNP sites in CDS of the orthologous gene pairs between Asia II 3 and MEAM1.

To investigate the regulatory potential of these distal regions showing methylation divergence, we analyzed the occurrence of 23 histone modifications around these 53 most discordant CpG sites.

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