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PTRG_11772 is the most diverged member of this family, yet the cysteine residues in all of the family members are conserved.
Interestingly, one of the 'classical' bld genes, bldB, encodes a diverged member of the SCO4542 family, but is an 'orphan' lacking neighbouring whiJ- or SCO4544-like genes.
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AnoACE8 and Apis 10228 form diverged members of this group.
C. elegans ACN-1 and vertebrate ACE2 proteins form highly diverged members of this family.
This phenomenon may indicate a functional redundancy with paralogous proteins or diverged members in C. elegans.
Note that slipin-1 [SLP-1] proteins are not included but are highly diverged members of the stomatin protein family.
It appears that choosing 200 PAM as the upper ceiling has not eliminated an important number of groups with highly diverged members.
A recent study by Fučíková et al. (2014) placed Mychonastes as the deepest diverging member of Sphaeropleales based on an analysis of four chloroplast genes.
Phylogenetic analyses of both datasets using MP and ML clearly indicate that the Vitaceae is sister to the remaining rosids, and therefore represents an early diverging member of the rosid clade.
However, although the overall character of the mt genome might support this hypothesis, the phylogenetic information coming from sequences of the mt genes favors the placement of Mychonastes well within Sphaeropleales, with Atractomorpha being the deepest diverging member of the order, consistent with analyses of nuclear ribosomal genes (Keller et al 2008; Fučíková et al. 2014).
All cyanobacterial genomes and, with one exception the recently sequenced Ignavibacterium album bacterium (an early diverging member of the chlorobia phylum) (Iino et al. 2010; Liu et al. 2012), all chlorobial genomes sequenced so far have genes coding for the photosynthetic apparatus.
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CEO of Professional Science Editing for Scientists @ prosciediting.com