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To perform this mapping, we use a single best-matching protein, which we refer to as the best target protein (BTP), rather than a collection of related proteins, to avoid including large gene families containing paralogs of possibly diverged function (see Methods).
Furthermore, though key conserved structure and sequence features point to a diverged TCS in M. pneumoniae, its divergence may lead also to diverged function (see examples above).
For example, we cannot assert that the dS/dN ratio for the YUB.2-MUS.A5 pais isignificantlyly different than neutrality (dS/dN = 1), a possibility if one or both genes have become cryptic pseudogenes (i.e., non-functional as opposed to diverged function).
There is likely to be some diverged function for the two genes as they have differences in expression pattern [88].
This pattern implies a likely diverged function of GmSEP4 with additional roles in the early floral initiation process as well as in nodule formation.
A recent study in Drosophila also showed that newly duplicated genes usually did not have alternative splicing isoforms and were expressed at a lower expression level, while those genes may have gained their diverged function or expression patterns after they developed alternative splicing activity [ 15].
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The utilization of biogas shows a number of diverged functions and has achieved a high level of industrialization and commercialization in OECD countries (GARR 2012; U.S.EPA 2015; Couture and Gagnon 2010; FIT 2012), such as electricity production, biogas vehicles, and other transport systems (SEA 2012; Fallde and Eklund 2015; Olsen et al. 2013; NIFU 2015; Gildas 2010; Bojesen et al. 2014).
We show that while the three different CRT family members in Arabidopsis have retained basic CRT functions, they have attained diverged functions in planta.
Arabidopsis VRN1 and Glyma11g13220 exhibit conserved as well as diverged functions.
We further investigated the possibly diverged functions of the four TRP-N paralogs in Hydra.
Furthermore, the differences in evolutionary pattern between gene families or species also suggest different evolutionary pressures and diverged functions.
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