The disturbance of expression of G1/S and G2/M phase cell cycle genes by NCS significantly affected the post-embryonic development of the Arabidopsis roots.
Clearly the role of TopBP1 in replication and genome maintenance would mean that disturbance of expression could result in genomic instability contributing towards cancer.
Further, small deletions resulting from ZFN-mediated non-homologous end joining (NHEJ) events are likely to cause little disturbance of expression of neighboring genes, whereas the conventional knockout technology often relies on the insertion of a selection marker, with a higher chance of a neighboring effect.
To check whether changes in mRNA level result in concomitant disturbances of protein expression we performed Western blot analysis of two splicing factors, SF2/ASF and hnRNP A1 on twelve representative pairs of control and tumor samples (Fig. 4).
Thus, disturbances of their expression can directly influence the progression of meiosis.
As seen in other cancers, pancreatic cancer also occurs due to genetic alterations and disturbances of gene expression [ 3].
It is possible that Ckap2l contributes to cell cycle regulation in HSCs and progenitors, and that genetic disturbances of its expression impact t-AML susceptibility.
Therefore, it comes of no surprise that disturbances of collagen expression is related to severe disorders, such as HANAC (Hereditary Angiopathy, Nephropathy, Aneurysms, and Cramps) syndrome, a COL4A1-related disorder that presents with retinal tortuosity and muscle cramps and with variable combinations of small vessel brain disease [ 56], or the "COL4A1 stroke syndrome" [ 81].
To further examine possible disturbance of gene expression in the Pygo1/ Pygo2 mutant kidneys, we used microarrays to perform a global analysis of gene expression changes.
In addition, MLN4924 exerted its suppressive effect on protein neddylation in cells, as indicated by a significant decrease in the expression of total Nedd8-conjugated (neddylated) proteins, cullin neddylation and by the increase in free Nedd8, without obvious disturbance of the expression of neddylation enzymes.
