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We sampled 52 field sites by genetic horizon to a 30 cm depth and determined particle size distribution, percent coarse fragments, Munsell color, and loss on ignition.
A more extensive description of the referent group is provided in Table 1, including the monthly distribution, percent of days with precipitation, and average wind speed.
To use both the staining intensity (graded 0, absent; 1, weak; 2, moderate; 3, strong) and distribution (percent cells staining) in the assessment of each core, we calculated an intensity score (percent cells staining × intensity of staining).
To study the dynamics of MP on the cell cycle, we incubated human T-cell leukaemia cell lines (Molt-4 sensitive and resistant subline and P12 resistant) with 10 μ M MP and measured total cell count, cell cycle distribution, percent viable, percent apoptotic, and percent dead cells serially over 72 h.
To measure total cell count, cell cycle distribution, percent viable, percent apoptotic, and percent dead cells, cells were stained with annexin-V and propidium iodide (PI) and analysed by flow cytometry (FACS) with the Coulter EPICS-V flow cytometer (Coulter Electronics Inc., Hialeah, FL, USA).
For the statistical evaluations in the survival experiments, we used the classical survival parameters including the survival distribution, percent survival at the end of the census period, median survival time (LT50), and the hazard ratio of death, using the Cox Proportional Regression analysis to generate the Wald Statistic.
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Data was analyzed with distribution percent-frequency.
Several proof-of-concept experiments with polycyclic aromatic hydrocarbons and fullerenes were performed and the results: product distributions, percent conversion of the starting material to useful products, etc., were compared to other synthetic methods.
Information about the distributions (percent > MDL, GMs, quartile cut points) of the chemicals and sums are presented in Supplemental Material, Table S1, and were described in detail previously (Windham et al. 2010).
In addition, descriptive statistics including the mean, standard deviation (SD) and score distributions (percent scoring at the best possible score or ceiling effect) were evaluated for new smoking-specific and SF-36 measures.
On the basis of natural breaks in the overall distribution of percent cropland in the study area, we assigned counties with a total percent cropland of < 20%percenttothethe referent category (n = 515).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com