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In addition to providing a single border strength metric by which any border may be assessed, our method enables analysis of the distribution of stability values ( s x, y ) for each border.
This type of calculation was initially introduced by Tokuriki et al. [30] to study the overall distribution of stability effects for all possible mutations in a large set of different single domain globular proteins.
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Though increased in stability by a uniform factor, D10A shows a distribution of stabilities in its secondary structural units that is similar to that of E. coli RNase H, but not the closely related protein from Thermus thermophilus.
In this case, we describe the distribution of stabilities by the column vector x t), with element x i (t) giving the fraction of proteins in stability bin i at time t.
The final obtained skewed distribution of Δ G was in good agreement with the empirical distribution of stabilities derived from the Protherm database (see the bottom panel in fig. 2 A) (Sarai et al. 2001).
The fraction of these single mutants that still fold is just eWy0, and the distribution of stabilities among the single mutants is y1 = Wy0 (note that the elements of y1 no longer sum to one).
Similarly, after m mutations, the fraction of mutants that still fold is eW m y0, and the distribution of stabilities among the m-mutants is y m = W m y0.
Using the FoldX algorithm that attempts to computationally predict ΔΔG effects of mutations, we deduced the overall distributions of stability effects for all possible mutations in 21 different globular, single domain proteins.
The effect of particle-size distribution on stability and performance of optimized Eco-SCC is evaluated.
HEK293 and H9 cells, two embryo-related cells, show one-peak distribution of miRNA stability skewed to low stability), whereas PC-3 M-IE8, T/G HA-VSMC, MCF-7, NSC, and Panc-1 cells show one-peak distribution skewed to high stability).
To examine a global stability of miRNAs and make a comparison across different cell types, we first analyzed the distribution of miRNA stability.
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