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The distribution of replication initiation events is responsive to local and global changes in chromatin structure and is affected by transcriptional activity.
This results in a broad distribution of replication fork rates in budding yeast [18].
What we were left with was a distribution of replication times, independent of sequence and length.
From the filtered replication times we could directly infer the distribution of replication rates, since all length-specificity is filtered out.
Differential timing in origin firing determines the number and distribution of replication forks along chromosomes and has important implications for genome stability.
A genome wide functional analysis of the distribution of replication origins in budding yeast has shown significant agreement with a computational analysis based solely on the distribution of ARS-related motins in the yeast genome[4], [5].
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In the present study, we were able to discern the distribution of replication-associated proteins, in the context of DNA replication, at the level of individual cells.
During acute infection, the distribution of viral replication differed from the distribution of GUSB enzyme expression.
A volcano-like shape illustrates the potential distribution of average-replication timings for all loci with various replication efficiencies (Figure 2E).
Eukaryotes have a distinct spatial and temporal pattern of genome replication that is dictated by the distribution and activity of replication origins.
The peculiar distribution of the DNA replication machinery has led to suggestions not only of a cenancestor endowed with an RNA genome, but also of the polyphyletic origins of DNA and many of enzymes associated with DNA replication (Leipe et al. 1999; Koonin and Martin 2005) in which viruses may have played a central role (Forterre, 2006).
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